DOT Follow-Up (Quest)
$85.00
DOT follow-up urine drug test through Quest Diagnostics for return-to-duty compliance. Required under 49 CFR Part 40 for employees returning after a DOT violation.

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The DOT Follow-Up test through Quest Diagnostics satisfies the mandatory follow-up testing requirements under 49 CFR Part 40 for safety-sensitive employees who have returned to duty after a substance use violation. Follow-up tests must be unannounced and follow a schedule determined by a Substance Abuse Professional (SAP).
DOT-Required Substances
- Marijuana (THC)
- Cocaine
- Opioids
- Amphetamines
- PCP
Compliance Requirements
- Minimum 6 tests in first 12 months after return to duty
- Unannounced and unpredictable scheduling required
- SAP directs testing frequency and duration
- Covers up to 5 years per DOT regulations
Lab Partner: Quest Diagnostics | Compliance: 49 CFR Part 40 | Results: 1-3 business days
Frequently asked questions
Negative urine and instant test results are typically available within 24 hours. Hair test results are delivered within 5–7 business days through USDTL or Quest/Labcorp. Positive results may require additional MRO confirmation and can take 48–72 hours. All results are delivered to Email.
Yes. All testing is 100% confidential and HIPAA compliant. Results are delivered exclusively to the ordering party through Email, our secure encrypted results portal. We do not share results with any third party without your authorization.
Yes. We offer employer programs including pre-employment testing, random testing pools, DOT consortium enrollment, and post-accident programs. Contact us at 615-332-8838 to discuss volume pricing and program setup.
Instant tests through the Quest ERO (Electronic Results Online) network deliver results at the collection site on the same day. They are available at Quest PSC sites and are ideal for situations where same-day decisions are needed. Negative results are available immediately; any non-negative results are confirmed by the lab.
Yes. We offer DOT-compliant urine drug testing that meets all requirements under 49 CFR Part 40 for FMCSA, FAA, FRA, FTA, PHMSA, and USCG regulated employers. DOT testing includes MRO review and is available through both Quest Diagnostics and Labcorp.
Urine tests detect recent drug use within the past 1–5 days for most substances. Hair tests detect use over a 90-day window and cannot be defeated by short-term abstinence. Hair testing through USDTL is the gold standard for pre-employment, probation, and custody applications. Urine testing via Quest and Labcorp is the standard for DOT compliance.
Customer Reviews
EXCELLENT
Based on 229 reviews
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Niah RTrustindex verifies that the original source of the review is Google.
Welcoming & kind staff! Quick & fast servicePosted on Google![]()
Ebone GTrustindex verifies that the original source of the review is Google.
Nisha the best, Few ppl was there as well she move quick and professionally and got through my drug testPosted on Google![]()
Madhusudhan GTrustindex verifies that the original source of the review is Google.
Great servicePosted on Google![]()
Lashonna PTrustindex verifies that the original source of the review is Google.
Friendly staff. Speedy service.Posted on Google![]()
Jonathan MTrustindex verifies that the original source of the review is Google.
Great overall experience and FAR BETTER, faster, and easier than any other facility I’ve been to. Best of all, the owner and staff were extremely friendly, understanding, and helpful. All around impeccable customer service. These are qualities are far to often under appreciated and many customers likely take them for granted. In my experience, these qualities did not go unnoticed and were much appreciated! Would highly recommend to any and all of those who are in need of their services!!Posted on Google![]()
Amanda WTrustindex verifies that the original source of the review is Google.
Awesome place for testing!!!!Posted on Google![]()
Kyra MTrustindex verifies that the original source of the review is Google.
Very kind staff clean facilityPosted on Google![]()
Darcy TTrustindex verifies that the original source of the review is Google.
Everybody was extremely nice and helpful’Posted on Google![]()
Jackie DTrustindex verifies that the original source of the review is Google.
I love Reliable labs so fast with good customer service got me in and out. Nesha was so good and nice thank you I will be backVerified by TrustindexTrustindex verified badge is the Universal Symbol of Trust. Only the greatest companies can get the verified badge who has a review score above 4.5, based on customer reviews over the past 12 months. Read more

